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opti mem  (Santa Cruz Biotechnology)


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  • 96

    Structured Review

    Santa Cruz Biotechnology opti mem
    Opti Mem, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+medium/siRNA+Transfection+Reagent/pm41830491-154-29-20
    Average 96 stars, based on 1966 article reviews
    opti mem - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Vertical RAS pathway inhibition in pancreatic cancer drives therapeutically exploitable mitochondrial alterations.
    Article Snippet: .. All siRNAs, the transfection reagent (sc-29528), and the transfection medium (sc-36868) were purchased from Santa Cruz Biotechnology. ..

    Article Title: A predictive mechanochemical modeling framework for the deformation and remodeling of the nuclear lamina
    Article Snippet: .. To induce lamin A/C depletion, U2OS cells were transfected with lamin A/C siRNA (sc-35776, Santa Cruz Biotechnology) using the siRNA Transfection Reagent (sc-29528) and Transfection Medium (sc-36868) according to the manufacturer’s protocol. ..

    Article Title: Vertical RAS pathway inhibition in pancreatic cancer drives therapeutically exploitable mitochondrial alterations
    Article Snippet: .. All siRNAs, the transfection reagent (sc-29528), and the transfection medium (sc-36868) were purchased from Santa Cruz Biotechnology. ..

    Article Title: Neuron-glioma synaptic transmission amplified by free 19S proteasome-mediated AMPAR deubiquitination promotes tumor progression.
    Article Snippet: .. In Tube A, 5 μL of siRNA was added to 100 μL of transfection medium (Santa Cruz Animal Health, sc-36868). .. In Tube B, 5 μL of transfection reagent (Santa Cruz Animal Health, sc-29528) was added to 100 μL of transfection medium.

    Article Title: Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo
    Article Snippet: .. A truncated β - catenin GATA6 inducible cell line was obtained by CRISPR mutation using ready-made plasmids from Santa Cruz (sc-419477) formed by three different gRNA (one: ATGAGCAGCGTCAAACTGCG; two: AGCTACTTGCTCTTGCGTGA; three: AAAATGGCAGTGCGCCTAGC). tet::Gata6 -mCherry mESCs were transfected chemically using the transfection reagent (Santa Cruz, sc-395739) and transfection medium (Santa Cruz, sc-108062). .. Transfected mESCs were sorted based on GFP expression using a FACS Aria (BD Biosciences) 8 hours after transfection.

    Article Title: Inflammatory Co-Regulation of Voltage-Gated Sodium Channels and Na,K-ATPase in Metastatic Breast Cancer
    Article Snippet: For Ringer’s experiments cells were washed in either Ringer’s buffer (Thermo Scientific, Waltham, MA, USA, AAJ67572AP) or sodium-free Ringer’s buffer (Thermo Scientific, AAJ67760AP), before being incubated in fresh Ringer’s buffer or sodium-free Ringer’s buffer for 30 min before adding experimental media. .. Transfection medium was prepared by combining siRNA against SCN5A (Santa Cruz, Dallas, TX, USA, SC-42640), ATP1A1 (IDT, Coralville, IA, USA, 259214281), or ATP1A3 (IDT, 259214675) with additive-free Opti-MEM (Gibco, 3185-070) and Lipofectamine 2000 transfection reagent (Invitrogen, Waltham, MA, USA, 11668-019). ..

    Article Title: Neuron-glioma synaptic transmission amplified by free 19S proteasome-mediated AMPAR deubiquitination promotes tumor progression
    Article Snippet: .. In Tube A, 5 μL of siRNA was added to 100 μL of transfection medium (Santa Cruz Animal Health, sc-36868). .. In Tube B, 5 μL of transfection reagent (Santa Cruz Animal Health, sc-29528) was added to 100 μL of transfection medium.

    Article Title: Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo.
    Article Snippet: .. A truncated β-catenin GATA6 inducible cell line was obtained by CRISPR mutation using ready-made plasmids from Santa Cruz (sc-419477) formed by three different gRNA (one: ATGAGCAGCGTCAAACTGCG; two: AGCTACTTGCTCTTGCGTGA; three: AAAATGGCAGTGCGCCTAGC). tet::Gata6-mCherry mESCs were transfected chemically using the transfection reagent (Santa Cruz, sc-395739) and transfection medium (Santa Cruz, sc-108062). .. Transfected mESCs were sorted based on GFP expression using a FACS Aria (BD Biosciences) 8 hours after transfection.

    CRISPR:

    Article Title: Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo
    Article Snippet: .. A truncated β - catenin GATA6 inducible cell line was obtained by CRISPR mutation using ready-made plasmids from Santa Cruz (sc-419477) formed by three different gRNA (one: ATGAGCAGCGTCAAACTGCG; two: AGCTACTTGCTCTTGCGTGA; three: AAAATGGCAGTGCGCCTAGC). tet::Gata6 -mCherry mESCs were transfected chemically using the transfection reagent (Santa Cruz, sc-395739) and transfection medium (Santa Cruz, sc-108062). .. Transfected mESCs were sorted based on GFP expression using a FACS Aria (BD Biosciences) 8 hours after transfection.

    Article Title: Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo.
    Article Snippet: .. A truncated β-catenin GATA6 inducible cell line was obtained by CRISPR mutation using ready-made plasmids from Santa Cruz (sc-419477) formed by three different gRNA (one: ATGAGCAGCGTCAAACTGCG; two: AGCTACTTGCTCTTGCGTGA; three: AAAATGGCAGTGCGCCTAGC). tet::Gata6-mCherry mESCs were transfected chemically using the transfection reagent (Santa Cruz, sc-395739) and transfection medium (Santa Cruz, sc-108062). .. Transfected mESCs were sorted based on GFP expression using a FACS Aria (BD Biosciences) 8 hours after transfection.

    Mutagenesis:

    Article Title: Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo
    Article Snippet: .. A truncated β - catenin GATA6 inducible cell line was obtained by CRISPR mutation using ready-made plasmids from Santa Cruz (sc-419477) formed by three different gRNA (one: ATGAGCAGCGTCAAACTGCG; two: AGCTACTTGCTCTTGCGTGA; three: AAAATGGCAGTGCGCCTAGC). tet::Gata6 -mCherry mESCs were transfected chemically using the transfection reagent (Santa Cruz, sc-395739) and transfection medium (Santa Cruz, sc-108062). .. Transfected mESCs were sorted based on GFP expression using a FACS Aria (BD Biosciences) 8 hours after transfection.

    Article Title: Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo.
    Article Snippet: .. A truncated β-catenin GATA6 inducible cell line was obtained by CRISPR mutation using ready-made plasmids from Santa Cruz (sc-419477) formed by three different gRNA (one: ATGAGCAGCGTCAAACTGCG; two: AGCTACTTGCTCTTGCGTGA; three: AAAATGGCAGTGCGCCTAGC). tet::Gata6-mCherry mESCs were transfected chemically using the transfection reagent (Santa Cruz, sc-395739) and transfection medium (Santa Cruz, sc-108062). .. Transfected mESCs were sorted based on GFP expression using a FACS Aria (BD Biosciences) 8 hours after transfection.



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